A COMPARISON OF FLUORESCENCE AND ULTRAVIOLET DETECTORS USING THE RP–HPLC TECHNIQUE FOR THE DETERMINATION OF TRAMADOL HYDROCHLORIDE (TRM-HCl): A CASE STUDY OF AL-BAYDA CITY, LIBYA

  • Noreldin S. Y. Abdolla Chemistry Department, Faculty of Science, Tobrouk University, Tobrouk, Libya and the current address: Chemistry Department, University of Leicester, UK, 11Corfield Rise, LE3 1QF
  • Suad K. Omar Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya
  • Ibrahim H. Habib National Research Centre, Applied Organic Chem. Dept., Dokki, Cairo, Egypt
  • Hana S. Mohamed Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya
Keywords: Tramadol, RP-HPLC, UV-D, FL-D, Sensitivity

Abstract

A fast and sensitive chromatographic RP–HPLC technique for examining tramadol hydrochloride (TRM-HCl) in tablets through using fluorescence detection (FL-D) and ultraviolet detection (UV-D) is reported in this paper. The separation was carried out using the reverse phase method on a Brownlee BIO C18 analytical column with a mobile phase consisting of 0.1% acetic acid and acetonitrile (2.5:7.5 v/v), which was pumped with an isocratic elution at a flow rate of 1 ml/min. The LOD and LQD values obtained in the current study indicate that FL-D is more sensitive, and hence preferable to UV detectors in the quantification of TRM-HCl tablets over the entire concentration range used (5-125 µg/ml). The study showed that the mean percentage recoveries from five samples were 99.93-100.023% (FL-D), which is somewhat similar to that of the UV-D (99.93-100.028). In conclusion, although Fl-D is acceptable for the quantification of tramadol tablets, UV-D offers higher detection sensitivity and reproducibility, particularly within concentrations that are low in the deposit collectors.

Author Biographies

Noreldin S. Y. Abdolla, Chemistry Department, Faculty of Science, Tobrouk University, Tobrouk, Libya and the current address: Chemistry Department, University of Leicester, UK, 11Corfield Rise, LE3 1QF

Chemistry Department, Faculty of Science, Tobrouk University, Tobrouk, Libya and the current address: Chemistry Department, University of Leicester, UK, 11Corfield Rise, LE3 1QF

Suad K. Omar, Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya

Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya

Ibrahim H. Habib, National Research Centre, Applied Organic Chem. Dept., Dokki, Cairo, Egypt

National Research Centre, Applied Organic Chem. Dept., Dokki, Cairo, Egypt

Hana S. Mohamed, Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya

Chemistry Department, Faculty of Science, Omar Al- Mukhtar University, Al- Bayda, Libya

Published
2020-09-27
How to Cite
Abdolla, N., Omar, S., Habib, I., & Mohamed, H. (2020). A COMPARISON OF FLUORESCENCE AND ULTRAVIOLET DETECTORS USING THE RP–HPLC TECHNIQUE FOR THE DETERMINATION OF TRAMADOL HYDROCHLORIDE (TRM-HCl): A CASE STUDY OF AL-BAYDA CITY, LIBYA. Scientific Journal of Applied Sciences of Sabratha University, (issue 5), 62-76. Retrieved from https://jas.sabu.edu.ly/index.php/asjsu/article/view/102