EFFECTS OF VINEGAR ACID ON THE DUODENUM TISSUES IN RATS. HISTOPATHOLOGICAL AND HISTOCHEMICAL STUDIES
The study aimed to investigate the histological and histochemical changes on the duodenum tissues induced by vinegar acid (VA) at a dose of 1 ml/kg/day (5 % and 10 %) in female rats for two weeks. Thirty rats were used, they were divided into three groups, the first group was given distilled water as the control group, the second group was given VA with a dose [1 ml/kg (5 %)] and the third group was given VA with a dose [1 ml/kg (10 %)] for two weeks. Histopathological statement of the duodenum tissues showed many vacuolations in epithelial cells, decrease in the number of goblet cells, erosion of mucosal epithelium, inflammatory cells infiltration in lamina propria, and submucosal oedema in all treated groups. Although that the histochemical study with PAS-reaction and bromophenol blue technique showed a decrease in the carbohydrate content and protein content in treated rats as compared with the control group. In conclusion, this study showed that vinegar acid was a harmful agent associated with histopathological changes that caused acute duodenum lesions and ulcers.