ANTIMICROBIAL RESISTANCE IN UROPATHOGEN ISOLATES FROM PATIENTS WITH URINARY TRACT INFECTIONS

Abstract

Because uncontrolled and wide spread use of antibiotics, the resistance pattern of uropathogens is changing drastically, specifically in developing countries, such as Libya. The aim of the study was to identify the common causes of urinary tract infection (UTI) causing pathogens in the city of Alzawiya, Libya and to check the performance of available antibiotics used by those patients. Study design: random 203 UTI patients who exhibited general UTI systems were included in current study. A questioner was prepared to input the information associated with the experiment including symptoms of disease, age, sex, laboratory diagnosis and antimicrobial susceptibility. Place and duration of study: Arriada laboratories, Alzawiya, Libya, between May 2023 and Dec 2023. Methodology: midstream clean-catch urine samples were collected from 203 suspected urinary tract infection patients of different age and sex groups. Uropathogens were identified by standard and specific microbiological techniques and antimicrobial susceptibility pattern was determined by Kirby Bauer Disc diffusion method following clinical and laboratory standards institute (CLSI) guidelines. findings: Sixty-one (30%) urine samples yielded significant bacteriuria of which 50 (82%) were obtained from female patients and 11 (18%) from male patients. Out of 61UTI patients, the prevalence of E. coli was 36 (59%) whereas for K. pneumonia was 18 (29.5%). Five were identified as Pseudomonas. aureuginosa while 2 were identified as Proteus. Mirabilis. most of the organisms were moderately resistant to Sulphamethoxazole, Cefuroxime, Cefixime and Nitrofurantion. E. coli showed 97.2% sucsebtibility to Amikacin (35/36, 97.2%) and resistant to Sulphamethoxazole (19/36, 52.7%). Klebsiella. Pneumonia was sucsebtible to Amikacin (18/18, 100%) and resistant to Sulphamethoxazole (11/18, 61.1%). P. aeuruginosa was found to show susceptibility to Ciprofloxacin and Amikacin (5/5, 100%) and 100% resistant to Cefuroxime (5/5, 100%). Proteus. Mirabilis found to be sucsebtible to Ciprofloxacin, Amikacin and Cefixime (2/2, 100%) and 100% resistant to Cefuroxime and Nitrofurantion (2/2, 100%). Conclusion: urinary tract infection has high prevalence in females than males in the area of study with most commonly bacterial isolates of E. coli and K.pneumonia. Although high percentage of uropathogens isolates developed a resistance against available used antibiotics, it seems that combined use of antibiotics is more effective against a particular uropathogen.