ENHANCEMENT OF FUNCTIONAL PROPERTIES AND BIOLOGICAL ACTIVITY IN BARLEY AND WHEAT GRAINS BY GERMINATION

  • Naji Elhadi Aborus University of Nalut, Faculty of Medical Technology, Nalut, Libya
  • Jasna Čanadanović Brunet University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia
  • Vesna Šaponjac University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia
  • Jelena Vulić University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia
Keywords: Cereal Sprouts, Phytochemicals, Free Radicals, Bioactivities

Abstract

In recent years, sprouted grains have become very popular and widely accepted as a functional food because of their nutritious and health benefits. So, this study was performed to evaluate the phytochemical com­position, and in vitro antioxidant capacity, reducing power, antihyperglycemic and anti-inflammatory activities, of seven-day old cereal sprouts (CS): Cultivars, barley NS565 (BSNS), barley Golozr­ni (BSG), wheat Spelta (WSSPE), wheat Simonida (WSSIM). Phenolic compounds were the most dominant bioactives in all CS. BSNS expressed significantly higher (p≤ 0.05) content of total phenols, chlo­rophyll and carotenoids. The total flavonoids content (TFC) in CS showed that barley sprouts (BSNS, BSG) had the higher value (P≤ 0.05) than wheat sprouts (WSSPE, WSSIM) respectively. The freeze-dried sprouts powders (FDSP) extracts were screened for possible antioxidant ca­pacities using DPPH, ABTS, and reducing power (Rp) assays. The results indicated that the BSNS possessed higher antioxi­dant capacities in DPPH and ABTS assays, and reducing power (IC50DPPH = 0.54 mg/ml; IC50ABTS = 0.79 mg/ml; IC0.5RP = 9.35 mg/ml) respectively. The inhibitory effect of FDSP extracts on α-glucosidase activity was investigated. The BSNS extracts exhibited higher inhibitory activity (IC50AHgA = 1.43 mg/ml) against α-glucosidase (p≤ 0.05). The anti-inflammato­ry activity (Denaturation of protein in vitro) showed signifi­cantly different between the FDSP, and Diclofenac sodium (DS). The IC50AIA of DS and BSNS was 0.79 and 1.86 (mg/ml) res­pectively. There was a strong positive correlation between TPC and antioxidant activities and reducing power, and also between TFC and anti-inflammatory activity.

Author Biographies

Naji Elhadi Aborus, University of Nalut, Faculty of Medical Technology, Nalut, Libya

University of Nalut, Faculty of Medical Technology, Nalut, Libya

Jasna Čanadanović Brunet, University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

Vesna Šaponjac, University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

Jelena Vulić, University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

University of Novi Sad, Faculty of Technology, Bulevar Cara Lazara 1, Novi Sad, Serbia

Published
2019-12-05
How to Cite
Aborus, N., Brunet, J., Šaponjac, V., & Vulić, J. (2019). ENHANCEMENT OF FUNCTIONAL PROPERTIES AND BIOLOGICAL ACTIVITY IN BARLEY AND WHEAT GRAINS BY GERMINATION. Scientific Journal of Applied Sciences of Sabratha University, 3(3), 20-41. Retrieved from https://jas.sabu.edu.ly/index.php/asjsu/article/view/55